期刊
DYES AND PIGMENTS
卷 197, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2021.109844
关键词
CPNs; AuNRs; Fluorescence platform; Hyaluronidase
资金
- National Natural Science Foundation of China [NSFC 51403111, 11774188]
- Shandong Provincial Natural Science Foundation [ZR2020MB062, ZR2017BH053]
- Universities Twenty Foundational Items of Jinan City [2019GXRC036]
- Joint Research Foundation for Young Doctorates of Qilu University of Technology (Shandong Academy of Sciences) [2019BSHZ0013]
A fluorescence platform based on the combination of conjugated polymer nanoparticles and gold nanorods was established for quantitative detection of hyaluronidase. The platform showed high selectivity and sensitivity, and was successfully applied to HAase quantification in a biological environment.
Hyaluronidase (HAase) is a momentous hydrolase related to many physiological and pathological processes. Therefore, it is urgent to establish a simple and accurate method for HAase detection. The combination of conjugated polymer nanoparticles (CPNs) and Au nanorods (AuNRs) provides an innovative method for platform design. In this work, a fluorescence platform was established for quantitative detection of HAase. Combined CPNs-NH2 and AuNRs using hyaluronic acid (HA) as the core by electrostatic adsorption, a novel turn on nanoplatform (CPNs-NH2-HA@ AuNRs) was established. The fluorescence of CPNs-NH2 will be quenched because of the fluorescence resonance energy transfer (FRET) between CPNs-NH2 and AuNRs. In the presence of HAase, AuNRs can be effectively decomposed, leading to the recovery of CPNs-NH2 fluorescence. The lower limit of detection of CPNs-NH2-HA@ AuNRs is obtained of 0.003 U/mL and the linear detection range is achieved within 1-45 U/mL. Furthermore, CPNs-NH2-HA@ AuNRs was applied in practice to the determination of HAase with high selectivity and sensitivity. With its superior performance, it was successfully applied to HAase quantification in biological environment. The experimental results show that CPNs-NH2-HA@ AuNRs is of great significance for the diagnosis of HAase related diseases.
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