期刊
DYES AND PIGMENTS
卷 198, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2021.109993
关键词
Carboxylesterases; Fluorescent probe; Near-infrared; Dicyanoisophorone; Living systems
资金
- Science and Technology Project of Henan Province [212102311021]
- Young Investigators Foundation of The First Affiliated Hospital of Zhengzhou University [YNQN2015192]
A new turn-on NIR fluorescent probe CE-1 with large Stokes shift was developed for accurate detection of carboxylesterases (CEs) with low cytotoxicity and high sensitivity. CE-1 was successfully used for cell imaging and real-time monitoring of CEs activity in mice models, showing potential application for imaging and monitoring endogenous CEs in living systems.
Accurate detection of carboxylesterases (CEs) plays a crucial role in various disease physiological processes and ester-containing drug metabolism. However, the reported near-infrared (NIR) fluorescent probes with short Stokes shift (<70 nm) limited their application of tracking CEs activity in complex bio-systems. Herein, a new turn-on NIR fluorescent probe for CEs detection (termed as CE-1) was developed based on dicyanoisophorone with large Stokes shift over 100 nm for the first time. The probe CE-1 (lambda ex/em = 535/665 nm) featured low cytotoxicity and high sensitivity (detection limit of 2.76 x 10-3 U/mL). Moreover, the CE-1 was successfully used to detect the level changes of CEs under drug-induced modulation in cell imaging, and real-time monitor the activity of CEs in mice models for biological imaging. Notably, these results indicate that the probe CE-1 can be applied for imaging and monitoring endogenous CEs in living systems. We believe that the probe CE-1 will be applied as a potential indicator of CEs for the clinical diagnosis of CEs-related diseases and rational use of drugs.
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