期刊
DYES AND PIGMENTS
卷 197, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2021.109930
关键词
Fluorescence; Near-infrared; Hydrogen peroxide; Bioimaging; Probe
资金
- Key Project of Hebei Science and Technology Department [21326612D]
- Key Projects of Hebei Educational Committee [ZD2020148]
- Basic Scientific Research Funds of Hebei Province [JYT2020006, JYT 2020010]
- Hebei Province Three Three Talent Project [A201901062]
- Youth Foundation of Hebei Educational Committee [QN2020402]
In this study, a large stokes shift and near-infrared probe DCM-HNU was designed and synthesized to visualize H2O2 in living cells and zebra fish, showing great potential for exploring the function and participation of H2O2 in biological systems.
In this work, we have designed and synthesized a large stokes shift and near-infrared (NIR) probe DCM-HNU for visualization of H2O2 in both living HeLa cells and zebra fish. The probe DCM-HNU was structured based on phenylborate pincinol ester as the recognition group connecting DCM-OH via nucleophilic substitution reaction. The probe possessed large stokes shift (205 nm), near-infrared (NIR) emission (658 nm). 0.17 mu M detection Limit, 10,550 Lmol- 1cm- 1 molar extinction coefficient, 2.06 x 10-3 S-1 reaction rate constant, and excellent selectivity proved the condition for practical application. There was a good linear relationship between fluorescence intensity and H2O2 concentration from 0 to 200 mu M (R2 = 0.9955) to facilitate quantitative analysis of H2O2. More importantly, the probe is able to visualize exogenous and endogenous H2O2 in living HeLa cells and zebra fish. The superior properties of the probe provide potential for exploring the function and participation of H2O2 in biological systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据