期刊
DIGESTIVE AND LIVER DISEASE
卷 54, 期 7, 页码 896-904出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.dld.2021.12.001
关键词
Circ-ABCB10; Gastric cancer; miR-1915-3p; Rac1; Tumorigenesis
资金
- Key research and development program of Shaanxi Province [2021SF-123]
This study revealed that circ-ABCB10 promotes GC progression by targeting the miR-1915-3p/Rac1 axis, suggesting that circ-ABCB10 could be a potential target for the diagnosis and treatment of GC.
Background: Gastric cancer (GC) is a common malignant tumor of the digestive system. Increasing reports have demonstrated the crucial roles of circRNAs in tumorigenesis and progression of GC. Methods: The relative expression of circ-ABCB10 in GC tissues and cell lines was detected by qRT-PCR. A series of in vitro assays and a xenograft model in vivo were applied to explore the function of circ-ABCB10 in GC cells. Results: Circ-ABCB10 expression was upregulated in GC tissues and cell lines and positively correlated with poor survival of GC patients. Circ-ABCB10 downregulation decreased cell viability, inhibited cell growth, invasion, and migration, while promoted cell apoptosis of GC cell lines SGC-7901 and MKN-48. Circ-ABCB10 could upregulate Rac1 expression by directly sponging miR-1915-3p. Rescue experiments revealed that miR-1915-3p inhibitor obviously reversed the inhibitory effect of si-circ-ABCB10, and Rac1 overexpression obviously reversed the inhibitory effect of miR-1915-3p mimics on cell growth, invasion, migration, apoptosis, and cell cycle progression. Moreover, si-circ-ABCB10 effectively inhibited tumor growth in a xenograft model. Conclusions: Our study revealed that circ-ABCB10 promoted GC progression via targeting the miR-1915-3p/Rac1 axis, and circ-ABCB10 might be a potential target for GC diagnosis and treatment. (C) 2021 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.
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