期刊
DIABETES RESEARCH AND CLINICAL PRACTICE
卷 184, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.diabres.2022.109189
关键词
Gut; Microbiome; Fungi; Mycobiome; Saccharomyces; Faecalibacterium prausnitzii; Pregnancy; Type 1 diabetes; ITS1; Inflammation
资金
- JDRF Australia
- Commonwealth of Australia grants for Accelerated Research under the Medical Research Future Fund
- Leona M. and Harry B. Helmsley Charitable Trust [key 3-SRA-2020-966-M-N]
- National Health and Medical Research Council of Australia (NHMRC) Centre of Research Excellence for the Protection of Pancreatic Beta Cells [1078106]
- NHMRC Program Grant [LCH 1150425]
- JDRF International [1-SRA-2018-543-S-B]
- Victorian State Government Operational Infrastructure Support scheme
- Diabetes South Australia
- NHMRC Research Institute Infrastructure Support Scheme
- NHMRC Practitioner Fellowship [1136735]
- LCH by an Investigator Fellowship [1173945]
The study reveals that pregnant women with type 1 diabetes experience changes in their gut fungal microbiome during pregnancy, accompanied by intestinal inflammation and impaired intestinal barrier function.
Aims: Studies of the gut microbiome have focused on its bacterial composition. We aimed to characterize the gut fungal microbiome (mycobiome) across pregnancy in women with and without type 1 diabetes. Methods: Faecal samples (n = 162) were collected from 70 pregnant women (45 with and 25 without type 1 diabetes) across all trimesters. Fungi were analysed by internal transcribed spacer 1 amplicon sequencing. Markers of intestinal inflammation (faecal calprotectin) and intestinal epithelial integrity (serum intestinal fatty acid binding protein; I-FABP), and serum antibodies to Saccharomyces cerevisiae (ASCA) were measured. Results: Women with type 1 diabetes had decreased fungal alpha diversity by the third trimester, associated with an increased abundance of Saccharomyces cerevisiae that was inversely related to the abundance of the anti-inflammatory butyrate-producing bacterium Faecalibacterium prausnitzii. Women with type 1 diabetes had higher concentrations of calprotectin, I-FABP and ASCA. Conclusions: Women with type 1 diabetes exhibit a shift in the gut mycobiome across pregnancy associated with evidence of gut inflammation and impaired intestinal barrier function. The relevance of these findings to the higher rate of pregnancy complications in type 1 diabetes warrants further study. (C) 2022 The Authors. Published by Elsevier B.V.
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