期刊
DEVELOPMENTAL BIOLOGY
卷 480, 期 -, 页码 105-113出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2021.08.011
关键词
RNAi; Echinoderm; Explants; Electroporation; Regeneration
资金
- NIH [R15GM124595]
- NSF [IOS-1252679]
- University of Puerto Rico
Regeneration is a vital developmental process where organisms recover tissue and organ components after injury or disease. The Wnt/β-catenin pathway plays a significant role in cell proliferation during intestine regeneration in sea cucumbers, as knocking down β-catenin transcripts reduces the number of proliferating cells but does not affect cell dedifferentiation or apoptosis. The development of an in vitro RNAi protocol is a crucial step in analyzing specific gene functions involved in echinoderm regeneration.
Regeneration is a key developmental process by which organisms recover vital tissue and organ components following injury or disease. A growing interest is focused on the elucidation and characterization of the molecular mechanisms involved in these regenerative processes. We have now analyzed the possible role of the Wnt/ beta-catenin pathway on the regeneration of the intestine in the sea cucumber Holothuria glaberrima. For this we have studied the expression in vivo of Wnt-associated genes and have implemented the use of Dicer-substrate interference RNA (DsiRNA) to knockdown the expression of beta-catenin transcript on gut rudiment explants. Neither cell dedifferentiation nor apoptosis were affected by the reduction of beta-catenin transcripts in the gut rudiment explants. Yet, the number of proliferating cells decreased significantly following the interference, suggesting that the Wnt/beta-catenin signaling pathway plays a significant role in cell proliferation, but not in cell dedifferentiation nor apoptosis during the regeneration of the intestine. The development of the in vitro RNAi protocol is a significant step in analyzing specific gene functions involved in echinoderm regeneration.
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