Contribution of Transcriptome to Elucidate the Biology of Plasmodium spp

The present review discusses some of the new technologies that have been applied to elucidate how Plasmodium spp escape from the immune system and subvert the host physiology to orchestrate the regulation of its biological pathways. Our manuscript describes how techniques such as microarray approaches, RNA-Seq, and single-cell RNA sequencing have contributed to the discovery of transcripts and changed the concept of gene expression regulation in closely related malaria parasite species. Moreover, the text highlights the contributions of high-throughput RNA sequencing for the current knowledge of malaria parasite biology, physiology, vaccine target, and the revelation of new players in parasite signaling.

Automated summary

This review discusses new technologies that have been used to understand how Plasmodium spp escape from the immune system and manipulate host physiology. The manuscript highlights the contributions of microarray approaches, RNA-Seq, and single-cell RNA sequencing in discovering transcripts and changing the concept of gene expression regulation in closely related malaria parasite species. It also emphasizes the role of high-throughput RNA sequencing in advancing our knowledge on malaria parasite biology, physiology, vaccine development, and identifying new players in parasite signaling.