期刊
CURRENT OPINION IN PLANT BIOLOGY
卷 65, 期 -, 页码 -出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.pbi.2021.102119
关键词
CRISPR; Tissue-specific; CRISPR-Activation; Gene silencing; Degron; Nanobodies; Conditional
资金
- FWO (Research Foundation e Flanders) [1174119N]
- ERC [639234, 864952]
- European Research Council (ERC) [864952, 639234] Funding Source: European Research Council (ERC)
Reverse genetics approaches are widely used for studying gene function, but severe mutations can limit functional studies in specific environments. The CRISPR technology has revolutionized targeted genome modification in plants, enabling inducible and tissue-specific manipulation of gene function at the DNA and RNA levels. Additionally, novel approaches for directly manipulating target proteins have been introduced in plant systems.
Reverse genetics approaches are routinely used to investigate gene function. However, mutations, especially in critical genes, can lead to pleiotropic effects as severe as lethality, thus limiting functional studies in specific contexts. Approaches that allow for modifications of genes or gene products in a specific spatial or temporal setting can overcome these limitations. The Palindromic Repeats) technologies has not only revolutionized targeted genome modification in plants but also enabled new possibilities for inducible and tissue-specific manipulation of gene functions at the DNA and RNA levels. In addition, novel approaches for the direct manipulation of target proteins have been introduced in plant systems. Here, we review the current development in tissue-specific and conditional manipulation approaches at the DNA, RNA, and protein levels.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据