期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 73, 期 -, 页码 198-204出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2021.08.010
关键词
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资金
- U.S. Department of Energy (DOE) [DE-SC0020420]
- National Science Foundation [MCB-1553721]
- Army Research Office [W911NF-19-1-0010]
- Institute for Collaborative Biotechnologies [W911NF-09-D-0001, W911NF-19-2-0026]
- US Army Research Office
- U.S. Department of Energy (DOE) [DE-SC0020420] Funding Source: U.S. Department of Energy (DOE)
Efficient utilization of lignocellulosic biomass requires the identification and engineering of new membrane proteins. Recent studies have discovered transporters sourced from anaerobic gut fungi, which enhance xylose transport and enable co-utilization of glucose and xylose. Additionally, fungal cellodextrin transporters have been identified as valuable alternatives for mitigating glucose repression and transporter inhibition.
Lignocellulose processing yields a heterogeneous mixture of substances, which are poorly utilized by current industrial strains. For efficient valorization of recalcitrant biomass, it is critical to identify and engineer new membrane proteins that enable the broad uptake of hydrolyzed substrates. Whereas glucose consumption rarely presents a bottleneck for cell factories, there is also a lack of transporters that allow co-consumption of glucose with other abundant biomass sugars such as xylose. This review discusses recent efforts to bioinformatically identify membrane proteins of high biotech potential for lignocellulose conversion and metabolic engineering in both model and nonconventional organisms. Of particular interest are transporters sourced from anaerobic gut fungi resident to large herbivores, which produce Sugars Will Eventually be Exported Transporters (SWEETs) that enhance xylose transport in the yeast Saccharomyces cerevisiae and enable glucose and xylose co-utilization. Additionally, recently identified fungal cellodextrin transporters are valuable alternatives to mitigate glucose repression and transporter inhibition.
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