期刊
CURRENT BIOLOGY
卷 32, 期 2, 页码 488-+出版社
CELL PRESS
DOI: 10.1016/j.cub.2021.11.063
关键词
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资金
- European Research Council under the Euro-pean Union (EU) 's Horizon 2020 research and innovation program [309858, 773153]
- Gatsby Charitable Foundation
- University of Zudrich
- Swiss National Science Foundation [31003A_182625]
- European Molecular Biology Organization (EMBO LTFs) [683-2018, 100-2017]
- Natural Sciences and Engineering Research Council of Canada [NSERC PDF-532561-2019]
- Biotech-nology and Biological Sciences Research Council
- National Science Foun-dation (BBSRC-NSF) [BB/V011294/1]
- Strategic Priority Research Program of the Chinese Acad-emy of Sciences [XDB27040204]
- Chinese 1000 Talents Program
- Shanghai Center for Plant Stress Biology
- Swiss National Science Foundation (SNF) [31003A_182625] Funding Source: Swiss National Science Foundation (SNF)
The availability of inorganic ortho-phosphate in soil is crucial for plant growth and fitness. This study reveals a link between plant immunity and phosphate homeostasis, showing that phosphate transport is repressed upon activation of immune responses. The repression is mediated by kinase-induced phosphorylation of the major phosphate transporter PHT1;4, and it negatively regulates anti-bacterial immunity in plant roots.
Soil availability of inorganic ortho-phosphate (PO43-, P-i) is a key determinant of plant growth and fitness.(1) Plants regulate the capacity of their roots to take up inorganic phosphate by adapting the abundance of H+-coupled phosphate transporters of the PHOSPHATE TRANSPORTER 1 (PHT1) family(2) at the plasma membrane (PM) through transcriptional and post-translational changes driven by the genetic network of the phosphate starvation response (PSR).(3-8) Increasing evidence also shows that plants integrate immune responses to alleviate phosphate starvation stress through the association with beneficial microbes.(9-11) Whether and how such phosphate transport is regulated upon activation of immune responses is yet uncharacterized. To address this question, we first developed quantitative assays based on changes in the electrical PM potential to measure active P-i transport in roots in real time. By inserting micro-electrodes into bulging root hairs, we were able to determine key characteristics of phosphate transport in intact Arabidopsis thaliana (hereafter Arabidopsis) seedlings. The fast P-i-induced depolarization observed was dependent on the activity of the major phosphate transporter PHT1;4. Notably, we observed that this PHT1;4-mediated phosphate uptake is repressed upon activation of pattern-triggered immunity. This inhibition depended on the receptor-like cytoplasmic kinases BOTRYTIS-INDUCED KINASE 1 (BIK1) and PBS1-LIKE KINASE 1 (PBL1), which both phosphorylated PHT1;4. As a corollary to this negative regulation of phosphate transport by immune signaling, we found that PHT1;4-mediated phosphate uptake normally negatively regulates anti-bacterial immunity in roots. Collectively, our results reveal a mechanism linking plant immunity and phosphate homeostasis, with BIK1/PBL1 providing a molecular integration point between these two important pathways.
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