Recent technological advances in mechanism, toxicity, and food perspectives of enzyme-mediated aflatoxin degradation

Aflatoxins are carcinogenic secondary metabolites produced by Aspergillus section Flavi that contaminates a wide variety of food and feed products and is responsible for serious health and economic consequences. Fermented foods are prepared with a wide variety of substrates over a long fermentation time and are thus vulnerable to contamination by aflatoxin-producing fungi, leading to the production of aflatoxin B1. The mitigation and control of aflatoxin is currently a prime focus for developing safe aflatoxin-free food. This review summarizes the role of major aflatoxin-degrading enzymes such as laccase, peroxidase, and lactonase, and microorganisms in the context of their application in food. A putative mechanism of enzyme-mediated aflatoxin degradation and toxicity evaluation of the degraded products are also extensively discussed to evaluate the safety of degradation processes for food applications. The review also describes aflatoxin-degrading microorganisms isolated from fermented products and investigates their applicability in food as aflatoxin preventing agents. Furthermore, a summary of recent technological advancements in protein engineering, nanozymes, in silico and statistical optimization approaches are explored to improve the industrial applicability of aflatoxin-degrading enzymes.

Automated summary

This review article summarizes the production and degradation of aflatoxins in food, focusing on major aflatoxin-degrading enzymes and microorganisms. It extensively discusses the mechanisms of enzyme-mediated aflatoxin degradation, toxicity evaluation of degraded products, and safety assessment of degradation processes for food applications. Additionally, recent technological advancements in protein engineering, nanozymes, and optimization approaches are explored to enhance the industrial applicability of aflatoxin-degrading enzymes.