期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
卷 251, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpc.2021.109192
关键词
Eriocheir sinensis; Icariin; Lipopolysaccharide; Oxidative stress; Mitochondrial apoptosis
资金
- Jiangsu Agriculture Science and Technology Innovation Fund (JASTIF) [CX (20) 3109]
- Jiangsu Agricultural Industry Technology System [JATS (2020) 423]
- National Natural Science Foundation of China [31802347]
- Guangdong key Areas R&D Programme Pro-jects [2020B0202010001]
This study found that dietary supplementation of 100 mg/kg of ICA enhanced the resistance of Eriocheir sinensis to oxidative damage and apoptosis induced by LPS challenge. It increased antioxidant capacity, reduced stress-related indicators, and alleviated cell apoptosis, along with regulating the expression of apoptosis-related genes and proteins.
To investigate the effects of dietary icariin (ICA) supplementation on acute oxidative stress and hepatopancreatic injury induced by lipopolysaccharide (LPS) injection in Eriocheir sinensis, an 8-week feeding trial of crabs was conducted using 4 diets with different supplementation levels of ICA (0, 50, 100, and 200 mg/kg diet weight, respectively), and then challenged with LPS of 400 mu g/kg body weight for 6 h. Results showed that 100 mg/kg ICA supplementation increased the antioxidant capacity, reduced the stress-related indicators in haemolymph, strengthen the mitochondrial membrane potential, and reduce apoptosis compared to the single LPS-treated crabs. The expressions of apoptosis-related genes and proteins were also evaluated to further understand the effects of dietary ICA pretreatment on LPS-induced cell apoptosis. As a result, dietary 100 mg/kg diet weight ICA pre-addition significantly down-regulated the expression of HSP60, HSP70, Caspase 3c, Caspase 8, Caspase 3, Caspase 9, P38, and Bax (P < 0.05), and alleviated the suppressed expression of PI3K, AKT, MEK, and Bcl-2 (P < 0.05) in crabs challenged with LPS. Overall, this research reveals that ICA supplementation of 100 mg/kg diet weight could enhance the resistance to oxidative damage and apoptosis in E. sinensis facing LPS challenge.
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