期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 208, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2021.112053
关键词
Low-density lipoprotein (LDL); Atherosclerosis; Atomic force microscopy (AFM); Oxidized LDL (oxLDL); Acidification
资金
- National Natural Science Foundation of China [81970386]
- Natural Science Foundation of Jiangxi Province of China [20172BCB22007]
Research shows that acidification causes changes in size and biomechanical properties of LDL/oxLDL particles, with significant decreases in hardness, potentially explaining their role in atherosclerosis.
Solution acidification exists under some physiological conditions (e.g. lysosomes in cells) and diseases (e.g. atherosclerosis, tumors, etc.). It is poorly understood whether and how acidification influences the size and biomechanical (stiffness and stickiness) properties of native Low-density lipoprotein (LDL) and its oxidized form (oxLDL) which plays a vital role in atherogenesis and tumorigenesis. Atomic force microscopy (AFM) evaluated that gradient acidification from pH 7.4 to pH 4.4 caused an expanding-first-and-then-shrinking decrease in size and a dramatic decrease in stiffness (but no statistically significant changes in stickiness) of LDL/oxLDL particles by influencing secondary/tertiary structures and lipid release detected by infrared spectral analysis and cholesterol detection, respectively. The smaller and softer characteristics of LDL/oxLDL at acidic conditions versus at the neutral pH partially explains the atherogenic role of acidification. The data may provide important information for a better understanding of LDL/oxLDL and some diseases (e.g. atherosclerosis and tumors).
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