期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 208, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2021.112034
关键词
Glucose oxidase; 3-Mercaptophenylboronic acid; ZIF-8; Glucose; Encapsulation
资金
- National Natural Science Foundation of China [21878131, 22078133, U1904174]
- Opening Project of Henan Province Key Laboratory of Water Pollution Control and Rehabilitation Technology [CJSZ2021002, CJSP2021001]
The composite 3-MPBA/GOx@ZIF-8 demonstrated high catalytic efficiency, loading efficiency, and enhanced stability, making it a potential candidate for biosensor preparation and diabetes screening.
Herein, a glucose oxidase@ZIF-8 composite (3-MPBA/GOx@ZIF-8) with enhanced enrichment was enabled the rapid encapsulation of glucose oxidase (GOx) into microporous zeolitic imidazolate framework-8 (ZIF-8) for the first time. The 3-MPBA/GOx@ZIF-8 not only has improved affinity and catalytic efficiency to the substrate but also can shorten the formation time. The optimum loading amount of GOx on ZIF-8 was determined to be 470 mg/g. The as-prepared 3-MPBA/GOx@ZIF-8 composite maintained the native conformation of the enzyme and showed excellent bioactivity, even in chemical agents or at high temperature. Furthermore, the 3-MPBA/ GOx@ZIF-8 showed satisfactory reusability, preserving almost 80.8 % activity after 7 cycles. The Michaelis constant Km and specificity constant kcat/Km of the 3-MPBA/GOx@ZIF-8 were 0.03 +/- 0.02 mM and 63.87 +/- 1.96 s- 1 mM-1, respectively, which were superior to corresponding values of free GOx. Therefore, the 3-MPBA/ GOx@ZIF-8 displayed high catalytic efficiency, high loading efficiency and enhanced stability. Moreover, a new type of visual colorimetric sensor for screening of the diabetes was realized through the 3-MPBA/GOx@ZIF8, which provided a new strategy for the analysis field of glucose.
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