Graphene oxide enriched with oxygen-containing groups: on the way to an increase of antioxidant activity and biocompatibility

The article is dedicated to the comprehensive biocompatibility investigation of synthesised graphene oxide (GO) enriched with oxygen-containing functional groups (similar to 85%). GO was synthesised through a modified Hummers and Offeman's method and characterised using C-13 NMR, Raman, and IR spectroscopy, XRD, HRTEM, along with size dimensions and zeta-potentials in aqueous dispersions. Biocompatibility study included tests on haemo-compatibility (haemolysis, platelet aggregation, binding to human serum albumin and its esterase activity), antioxidant activity (2,2-diphenyl-1-picrylhydrazyl reaction, NO-radical uptake, Radachlorin photobleaching, photo-induced haemolysis), genotoxicity using DNA comet assay, as well as metabolic activity and proliferation of HEK293 cells.

Automated summary

This article investigates the comprehensive biocompatibility of synthesised graphene oxide enriched with oxygen-containing functional groups. The study includes tests on haemocompatibility, antioxidant activity, genotoxicity, and metabolic activity of cells.