Modulation of laccase catalysed oxidations at the surface of magnetic nanoparticles

We explored the coupling of laccases to magnetic nanoparticles (MNPs) with different surface chemical coating. Two laccase variants offering two opposite and precise orientations of the substrate oxidation site were immobilised onto core-shell MNPs presenting either aliphatic aldehyde, aromatic aldehyde or azide functional groups at the particles surface. Oxidation capabilities of the six-resulting laccase-MNP hybrids were compared on ABTS and coniferyl alcohol. Herein, we show that the original interfaces created differ substantially in their reactivities with an amplitude from 1 to > 4 folds depending on the nature of the substrate. Taking enzyme orientation into account in the design of surface modification represents a way to introduce selectivity in laccase catalysed reactions.

Automated summary

The study explored the immobilization of laccase onto magnetic nanoparticles with different surface chemical coatings, which showed substantial differences in reactivity depending on the nature of the substrate, with amplitudes ranging from 1 to > 4 folds. Considering enzyme orientation in surface modification design represents a way to introduce selectivity in laccase-catalyzed reactions.