期刊
CLINICAL INFECTIOUS DISEASES
卷 73, 期 -, 页码 S325-S335出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/cid/ciab784
关键词
antimicrobial resistance; epidemiology; genomic surveillance; Klebsiella; Pathogenwatch
资金
- Official Development Assistance (ODA) funding from the National Institute for Health Research [16_136_111]
- National Institute for Health Research using ODA funding
Pathogenwatch is a web application designed for real-time analysis of Klebsiella genomes, aiding surveillance at local, national, and global levels. The platform allows for contextualization of user genomes with public databases, revealing the distribution of K. pneumoniae clones in different countries through phylogenetic analyses.
Background. Klebsiella species, including the notable pathogen K. pneumoniae, are increasingly associated with antimicrobial resistance (AMR). Genome-based surveillance can inform interventions aimed at controlling AMR. However, its widespread implementation requires tools to streamline bioinformatic analyses and public health reporting. Methods. We developed the web application Pathogenwatch, which implements analytics tailored to Klebsiella species for integration and visualization of genomic and epidemiological data. We populated Pathogenwatch with 16 537 public Klebsiella genomes to enable contextualization of user genomes. We demonstrated its features with 1636 genomes from 4 low- and middle-income countries (LMICs) participating in the NIHR Global Health Research Unit (GHRU) on AMR. Results. Using Pathogenwatch, we found that GIIRU genomes were dominated by a small number of epidemic drug-resistant clones of K. pneumoniae. However, differences in their distribution were observed (eg, ST258/512 dominated in Colombia, ST231 in India, ST307 in Nigeria, ST147 in the Philippines). Phylogenetic analyses including public genomes for contextualization enabled retrospective monitoring of their spread. In particular, we identified hospital outbreaks, detected introductions from abroad, and uncovered clonal expansions associated with resistance and virulence genes. Assessment of loci encoding O-antigens and capsule in K. pneumoniae, which represent possible vaccine candidates, showed that 3 O-types (O1-O3) represented 88.9% of all genomes, whereas capsule types were much more diverse. Conclusions. Pathogenwatch provides a free, accessible platform for real-time analysis of Klebsiella genomes to aid surveillance at local, national, and global levels. We have improved representation of genomes from GI IRU participant countries, further facilitating ongoing surveillance.
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