4.7 Article

HPV Sequencing Facilitates Ultrasensitive Detection of HPV Circulating Tumor DNA

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HPV ctDNA detection of high-risk HPV types during chemoradiotherapy for locally advanced cervical cancer

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Summary: The study designed HPV droplet digital PCR (ddPCR) assays for blood detection and monitored blood HPV circulating tumor DNA (HPV ctDNA) levels in patients with LACC undergoing CRT. Results showed successful detection of HPV ctDNA in 69% of patients before CRT, with a correlation between HPV-ctDNA levels and HPV copy number in tumors. Detection of HPV-ctDNA before CRT was associated with tumor stage and lymph node status, while positive detection during follow-up was linked to lower disease-free and overall survival rates.

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