4.6 Article

Unlocking the Hydrolytic Mechanism of GH92 α-1,2-Mannosidases: Computation Inspires the use of C-Glycosides as Michaelis Complex Mimics

期刊

CHEMISTRY-A EUROPEAN JOURNAL
卷 28, 期 14, 页码 -

出版社

WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.202200148

关键词

carbohydrates; conformations; enzymology; inhibitors; quantum mechanics

资金

  1. European Union's Horizon 2020 research and innovation programme under the Marie Skodowska-Curie grant [847548]
  2. UCT Prague [A1_FPBT_2021_002]
  3. Gilead Sciences, Inc.
  4. Ministry of Education, Youth and Sports of the Czech Republic [LTAUSA18085]
  5. Japan Society for the Promotion of Science KAKENHI [19K15748]
  6. Photon Factory Program Advisory Committee [2019G097]
  7. Grants-in-Aid for Scientific Research [19K15748] Funding Source: KAKEN

向作者/读者索取更多资源

Understanding the conformational changes in sugar moieties is important for studying glycoside hydrolase mechanisms and designing inhibitors. In this study, the conformational pathway of GH92 enzymes was predicted using computational methods and confirmed with experiments. The study also demonstrated the potential of a new type of C-disaccharides for biochemical applications.
The conformational changes in a sugar moiety along the hydrolytic pathway are key to understand the mechanism of glycoside hydrolases (GHs) and to design new inhibitors. The two predominant itineraries for mannosidases go via S-O(2)-> B-2,B-5 -> S-1(5) and S-3(1)-> H-3(4)-> C-1(4). For the CAZy family 92, the conformational itinerary was unknown. Published complexes of Bacteroides thetaiotaomicron GH92 catalyst with a S-glycoside and mannoimidazole indicate a C-4(1)-> H-4(5)/S-1(5)-> S-1(5) mechanism. However, as observed with the GH125 family, S-glycosides may not act always as good mimics of GH's natural substrate. Here we present a cooperative study between computations and experiments where our results predict the E-5 -> B-2,B-5/S-1(5)-> S-1(5) pathway for GH92 enzymes. Furthermore, we demonstrate the Michaelis complex mimicry of a new kind of C-disaccharides, whose biochemical applicability was still a chimera.

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