期刊
CHEMICAL PAPERS
卷 76, 期 2, 页码 879-888出版社
SPRINGER INT PUBL AG
DOI: 10.1007/s11696-021-01906-2
关键词
Amino acids; NMR spectroscopy; Chemical transformation; H-1 NMR; Kinetics; Decarboxylation
资金
- Projekt DEAL
This study examined the competence of amino acids as standards for instrumental biochemical analysis by using benchtop NMR spectrometer and mass spectrometry. It found that stability of amino acid standards varies under different conditions, with some undergoing chemical transformations.
In this paper, we examined the competence of amino acids as standards for instrumental biochemical analysis. The chosen amino acids were first dissolved in various aquatic solutions and then measured in a benchtop NMR spectrometer, which is not a common choice in such analytical investigations. Analysis by mass spectrometry was used in addition. As part of these investigations, we examined and determined the stability of the amino acids ornithine, glutamic acid, alanine, glycine, proline, pyroglutamic acid, phenylalanine and trans-4-hydroxy-D-proline under critical basic and acidic pH conditions and under various other conditions. We observed that not all solutions of the amino acid standards remain stable under the given conditions and a chemical transformation takes place. Given our findings by mass spectroscopy, additional kinetic measurements were carried out with the benchtop NMR spectrometer. We discovered that pyroglutamic acid becomes unstable under basic conditions and decarboxylates to pyrrolidone.
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