期刊
CHEMICAL ENGINEERING JOURNAL
卷 430, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2021.132664
关键词
Multifunctional hydrogel; Exosomes; Diabetic wound healing; Angiogenesis; Macrophage polarization
资金
- Military Logistics Scientific Research Program of China [BKJ18J003]
- National Natural Science Foundation of China [51973172]
- Natural Science Foundation of Shaanxi Province [2020JC-03, 2019TD-020]
This study developed a self-healing hydrogel capable of stably releasing exosomes to enhance diabetic wound healing. Through the synergistic effect of exosome release and M2 macrophage polarization, it accelerated angiogenesis and wound healing.
Developing multifunctional wound dressings capable of inducing rapid angiogenesis provides promise for diabetic wound healing, and triggering proper immune responses has been proposed as a potential way to facilitate angiogenesis. Here, we developed exosomes-laden injectable hydrogel with self-healing ability based on chitosan-graft-aniline tetramer (CS-AT) and dibenzaldehyde-terminated poly (ethylene glycol) (PEG-DA), which can enhance diabetic wound healing by stably releasing exosomes. In vivo, CS-AT hydrogel loaded with exosomes (CS-AT-Exo hydrogel) apparently accelerated the diabetic full-thickness wound healing, characterized by promoted wound healing efficiency, quick re-epithelization, favorable collagen deposition, and abundant angiogenesis at the wound beds. In addition, the synergistic effect of hydrogel and exosomes induced local regulation of M2 macrophages polarization. In vitro, CS-AT hydrogel and exosomes can synergistically promote the polarization of M2 macrophages in CS-AT-Exo hydrogel. Furthermore, the CS-AT-Exo hydrogel with coculture of macrophages significantly enhanced the ability of human umbilical vein endothelial cells (HUVECs) to proliferation, migration, and tube formation. These findings revealed important mechanisms by which multifunctional CS-AT-Exo hydrogels with continuous exosomes release can enhance angiogenesis and diabetic wound healing by synergistically inducing M2 macrophage polarization, suggesting a potential treatment strategy for diabetic wounds.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据