期刊
CHEMICAL ENGINEERING JOURNAL
卷 427, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2021.130913
关键词
Electrospinning; Aptamer; Box-Behnken Design strategy; Tetracycline; NH2-MIL-101(Fe)
资金
- National Key Research and Development Project [2019YFC0408304]
- Fundamental Research Funds for the Central Universities [2232020G-04]
- Fundamental Research Funds for the Central Universities and Graduate Student Innovation Fund of Donghua University [CUSF-DH-D-2021037]
- Key Laboratory of Jiangxi Province for Persistent Pollutants Control and Resources Recycle [ES201980203]
- China Scholarship Council (CSC)
The novel electrochemical aptasensor based on Fe-based MOFs was successfully fabricated to enhance sensitivity in detecting antibiotics; NH2-MIL-101(Fe)/CNF@AuNPs was synthesized to amplify electrical signals through the attachment of aptamers via Au-S bonds; By optimizing detection conditions and achieving quantitative detection of tetracycline in real water samples, the sensor exhibited good selectivity and stability, showing promise as a new strategy for detecting tetracycline in environmental water samples.
In this work, a novel electrochemical aptasensor based on Fe-based MOFs was fabricated. To enhance sensitivity to the detection of antibiotics, NH2-MIL-101(Fe)/CNF@AuNPs was synthesized by a combination of hydrothermal, electrospinning, pyrolysis and electrodeposition methods. Subsequently aptamer could be attached to the NH2-MIL-101(Fe)/CNF@AuNPs through Au-S bonds. The electrical signals generated from the interaction between antibiotics and aptamers were amplified by the NH2-MIL-101(Fe)/CNF@AuNPs aptasensor, resulting in a significant current/impedance response. Thus, signal generation and amplification were successfully realized. In addition, the Box-Behnken Design (BBD) strategy was adopted to optimize the detection conditions and greatly reduce experimental errors. Under optimized conditions, the tetracycline concentration detected by the sensor had a linear relationship with the impedance in the range of 0.1-105 nM TC. The lowest detection limit was 0.01 nM. NH2-MIL-101(Fe)/CNF electrospinning aptasensor exhibited good selectivity and stability. Furthermore, quantitative detection of tetracycline was achieved in real water samples. The sensor reported here could become a promising new strategy for detecting tetracycline in environmental water samples in the future.
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