期刊
CHEMICAL COMMUNICATIONS
卷 58, 期 18, 页码 2979-2982出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1cc06611k
关键词
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资金
- Shanghai Natural Science Fund [20ZR1414700]
- Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission) [2021 Sci Tech 03-28]
- Shanghai Sailing Program [19YF1412500]
- National Natural Science Foundation of China [21577037, 21738002, 21906057]
- State Key Laboratory of Bioreactor Engineering
- Ministry of Education, Singapore [MOE-MOET2EP10120-0007]
A highly selective and sensitive fluorescent probe (COU-LIP-1) was developed to monitor labile iron(II) pools (LIP) using coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive and fluorescence quenching group. The probe showed a turnon response through a photo-induced photon transfer (PET) mechanism upon Fe(II)-induced reductive cleavage of the N-O bond. It was successfully applied for monitoring labile iron(II) changes in macrophage activations and erastin-induced ferroptosis, providing a powerful tool for selectively sensing LIP under different conditions.
A fluorescent probe (COU-LIP-1) for monitoring labile Fe(II) pools (LIP) with high selectivity and sensitivity was developed utilizing coumarin 343 as the fluorophore and 3-nitrophenylazanyl ester as both the reactive group and the fluorescence quenching group. Fe(II)-induced reductive cleavage of the N-O bond results in a turnon response via a photo-induced photon transfer (PET) mechanism. The probe was applied for monitoring labile iron(II) changes in M1 and M2a macrophage activations and also erastin-induced ferroptosis, providing a powerful tool for selectively sensing LIP under both physiological and stressed conditions.
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