Bottom-up/cross-linking mass spectrometry via simplified sample processing on anion-exchange solid-phase extraction spin column

We introduce a simple single-column protein digestion method for low-microgram-level samples containing sodium dodecyl sulfate and Coomassie dye that can be completed within a few hours.

Automated summary

This method introduced is simple and suitable for low-microgram level samples containing sodium dodecyl sulfate and Coomassie dye, and can be completed within a few hours.