期刊
CHEMBIOCHEM
卷 23, 期 8, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.202200108
关键词
4-phenylbutan-2-amine; alcohol amination; biocatalysis; chiral amines; racemic 4-phenyl-2-butanol
资金
- H2020 ERACoBioTech programme through the Biotechnology and Biological Sciences Research Council [61 HOMBIOCAT, BB/R021287/1]
- Universitat Bern
The study presents a one-pot enzymatic cascade for the direct conversion of racemic alcohols into enantiopure chiral amines with high enantioselectivities. This method allows for the efficient transformation of waste reagents into valuable products, with in-situ cofactor recycling.
The synthesis of enantiopure chiral amines from racemic alcohols is a key transformation in the chemical industry, e. g., in the production of active pharmaceutical ingredients (APIs). However, this reaction remains challenging. In this work, we propose a one-pot enzymatic cascade for the direct conversion of a racemic alcohol into either (S)- or (R)-enantiomers of the corresponding amine, with in-situ cofactor recycling. This enzymatic cascade consists of two enantio-complementary alcohol dehydrogenases, both NADH and NADPH oxidase for in-situ recycling of NAD(P)(+) cofactors, and either (S)- or (R)-enantioselective transaminase. This cell-free biocatalytic system has been successfully applied to the conversion of racemic 4-phenyl-2-butanol into the high value (S)- or (R)-enantiomers of the amine reaching good (73 % (S)) and excellent (>99 % (R)) enantioselectivities.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据