期刊
CHEMBIOCHEM
卷 22, 期 24, 页码 3462-3468出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.202100433
关键词
bacterial imaging; fluorescent imaging; mecA methicillin-resistant S; aureus; peptidoglycans; transglycosylases
资金
- Ministry of Science and Technology
- Academia Sinica Thematic projects [AS-106-TP-L04-3, AS-106-TP-L04-4]
- Academia Sinica
Fluorescent probes play a crucial role in imaging bacterial PGN, especially for labeling key PGN-synthesizing enzymes like TGases. This study successfully synthesized the first imaging probe for labeling TGase, providing a new method for monitoring bacterial growth and division cycles, as well as studying cell wall growth when conventional probes are not applicable.
The imaging of peptidoglycan (PGN) dynamics in living bacteria facilitates the understanding of PGN biosynthesis and wall-targeting antibiotics. The main tools for imaging bacterial PGN are fluorescent probes, such as the well-known PGN metabolic labeling probes. However, fluorescent small-molecule probes for labeling key PGN-synthesizing enzymes, especially for transglycosylases (TGases), remain to be explored. In this work, the first imaging probe for labeling TGase in bacterial cell wall studies is reported. We synthesized various fluorescent MoeA-based molecules by derivatizing the natural antibiotic moenomycin A (MoeA), and used them to label TGases in living bacteria, monitor bacterial growth and division cycles by time-lapse imaging, and study cell wall growth in the mecA-carrying methicillin-resistant Staphylococcus aureus (MRSA) strains when the beta-lactam-based probes were unsuitable.
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