期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 79, 期 2, 页码 -出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-022-04154-z
关键词
Immune activation; T cell signaling; Immune suppression; TNFAIP3; TNIP1; CARMA1
资金
- Projekt DEAL
- Deutsche Forschungsgemeinschaft [SFB1054, 210592381, SFB1335, 360372040, 210879364]
- Carl-Zeiss-Stiftung
This study found that ABIN-1 modulates the suppressive function of A20 in T cells, and revealed that the A20/ABIN-1 module regulates CBM complex signaling and T cell activation through the interaction between A20 ZnF4/7 and the CBM complex.
T cell activation initiates protective adaptive immunity, but counterbalancing mechanisms are critical to prevent overshooting responses and to maintain immune homeostasis. The CARD11-BCL10-MALT1 (CBM) complex bridges T cell receptor engagement to NF-kappa B signaling and MALT1 protease activation. Here, we show that ABIN-1 is modulating the suppressive function of A20 in T cells. Using quantitative mass spectrometry, we identified ABIN-1 as an interactor of the CBM signalosome in activated T cells. A20 and ABIN-1 counteract inducible activation of human primary CD4 and Jurkat T cells. While A20 overexpression is able to silence CBM complex-triggered NF-kappa B and MALT1 protease activation independent of ABIN-1, the negative regulatory function of ABIN-1 depends on A20. The suppressive function of A20 in T cells relies on ubiquitin binding through the C-terminal zinc finger (ZnF)4/7 motifs, but does not involve the deubiquitinating activity of the OTU domain. Our mechanistic studies reveal that the A20/ABIN-1 module is recruited to the CBM complex via A20 ZnF4/7 and that proteasomal degradation of A20 and ABIN-1 releases the CBM complex from the negative impact of both regulators. Ubiquitin binding to A20 ZnF4/7 promotes destructive K48-polyubiquitination to itself and to ABIN-1. Further, after prolonged T cell stimulation, ABIN-1 antagonizes MALT1-catalyzed cleavage of re-synthesized A20 and thereby diminishes sustained CBM complex signaling. Taken together, interdependent post-translational mechanisms are tightly controlling expression and activity of the A20/ABIN-1 silencing module and the cooperative action of both negative regulators is critical to balance CBM complex signaling and T cell activation.
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