期刊
CELL DEATH AND DIFFERENTIATION
卷 29, 期 8, 页码 1474-1485出版社
SPRINGERNATURE
DOI: 10.1038/s41418-022-00937-w
关键词
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资金
- Ministry of Education of Spain (FPI grant) [BES-2016-077901]
- MINECO/AEI/FEDER (EU) [PID2019-104763RB-I00]
- Ramon y Cajal contract from MINECO/AEI/FEDER (EU) [RYC-2014-15991]
- European Commission [ERA-NET NEURON8-Full-815-094]
- AEI-MICIU/FEDER [RTI2018-095582-B-I00, RED2018-102723T]
- iLUNG programme from the Comunidad de Madrid [B2017/BMD-3884]
This study reveals that the centrosomal kinase PLK1 regulates centrosome asymmetry and cell fate in neural progenitors. Loss of PLK1 activity leads to reduced asymmetry and increased expansion of neural progenitors, promoting cortical growth. However, deficiencies in MCPH proteins result in increased centrosome asymmetry and microcephaly.
Alteration of centrosome function and dynamics results in major defects during chromosome segregation and is associated with primary autosomal microcephaly (MCPH). Despite the knowledge accumulated in the last few years, why some centrosomal defects specifically affect neural progenitors is not clear. We describe here that the centrosomal kinase PLK1 controls centrosome asymmetry and cell fate in neural progenitors during development. Gain- or loss-of-function mutations in Plk1, as well as deficiencies in the MCPH genes Cdk5rap2 (MCPH3) and Cep135 (MCPH8), lead to abnormal asymmetry in the centrosomes carrying the mother and daughter centriole in neural progenitors. However, whereas loss of MCPH proteins leads to increased centrosome asymmetry and microcephaly, deficient PLK1 activity results in reduced asymmetry and increased expansion of neural progenitors and cortical growth during mid-gestation. The combination of PLK1 and MCPH mutations results in increased microcephaly accompanied by more aggressive centrosomal and mitotic abnormalities. In addition to highlighting the delicate balance in the level and activity of centrosomal regulators, these data suggest that human PLK1, which maps to 16p12.1, may contribute to the neurodevelopmental defects associated with 16p11.2-p12.2 microdeletions and microduplications in children with developmental delay and dysmorphic features.
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