期刊
CELL CYCLE
卷 20, 期 20, 页码 2149-2159出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15384101.2021.1971352
关键词
Retinoblastoma; long non-coding rna prox1-antisense rna1; microrna-519d-3p; sex determining region y-box 2; proliferation; apoptosis
类别
In this study, PROX1-AS1 was found to regulate chemosensitivity in retinoblastoma by sponging miR-519d-3p and targeting SOX2. Inhibition of PROX1-AS1 or upregulation of miR-519d-3p suppressed the growth and invasion of drug-resistant cells, while promoting apoptosis.
Objective Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) participate in tumor progression, while the role of PROX1-antisense RNA1 (PROX1-AS1) sponging miR-519d-3p in retinoblastoma (RB) remains largely unknown. We aim to explore the effect of the PROX1-AS1/miR-519d-3p/sex determining region Y-box 2 (SOX2) in chemosensitivity of RB cells. Methods Expression of PROX1-AS1, miR-519d-3p and SOX2 in RB tissues and cells was determined. The drug-resistant cell lines were established and respectively intervened with PROX1-AS1 or miR-519d-3p expression to explore their roles in drug resistance and malignant behaviors of the drug-resistant cells. The binding relationships between PROX1-AS1 and miR-519d-3p, and between miR-519d-3p and SOX2 were evaluated. Results PROX1-AS1 and SOX2 were upregulated while miR-519d-3p was downregulated in RB tissues and cells, especially in drug-resistant cells. The PROX1-AS1 inhibition or miR-519d-3p elevation suppressed the drug resistance, proliferation, migration and invasion, and promoted apoptosis of the drug-resistant RB cells. Moreover, PROX1-AS1 sponged miR-519d-3p and miR-519d-3p targeted SOX2. Conclusion PROX1-AS1 knockdown upregulates miR-519d-3p to promote chemosensitivity of RB cells via targeting SOX2.
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