期刊
CELL CALCIUM
卷 100, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ceca.2021.102481
关键词
TRPV6; Genetic labeling; Cre recombinase; Mass spectrometry; Reproductive system; Teeth; Tongue; Salivary gland; Pancreas; Duodenum; Jejunum; Ileum; Cecum; Bladder; Bone; Uterus; Epididymis; Prostate
类别
资金
- Deutsche Forschungsgemeinschaft (DFG) [SFB Transregio TRR152, SFB 894, DFG WE 4866/1-1, DFG FE 629/2-1]
The study identified the widespread expression of TRPV6 channel throughout the body and provided a detailed analysis of its expression using various experimental methods, which will serve as an important reference for future research on the role of TRPV6 channels in vivo.
The transient receptor potential vanilloid 6 (TRPV6) channel is highly Ca2+-selective and has been implicated in mediating transcellular Ca2+ transport and thus maintaining the Ca2+ balance in the body. To characterize its physiological function(s), a detailed expression profile of the TRPV6 channel throughout the body is essential. Capitalizing on a recently established murine Trpv6-reporter strain, we identified primary TRPV6 channel-expressing cells in an organism-wide manner. In a complementary experimental approach, we characterized TRPV6 expression in different tissues of wild-type mice by TRPV6 immunoprecipitation (IP) followed by mass spectrometry analysis and correlated these data with the reporter gene expression. Taken together, we present a TRPV6 expression atlas throughout the entire body of juvenile and adult mice, providing a novel resource to investigate the role of TRPV6 channels in vivo.
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