期刊
CELL
卷 184, 期 23, 页码 5775-+出版社
CELL PRESS
DOI: 10.1016/j.cell.2021.10.014
关键词
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资金
- HHMI Gilliam Fellowship
- NSF GRFP Fellowship
- NIH [5 T32 GM 7616-40, NRSA CA247447, U01 DA040612, U01 HL130007]
- UCLA-Caltech Medical Scientist Training Program
- American Cancer Society Fellowship
- Caltech BBE fellowship
- NHLBI [F30-HL136080]
- USC MD/PhD Pro-gram
- Caltech Beckman Institute
- Arnold and Mabel Beck-man Foundation
- NYSCF
- Sontag Foundation
- Searle Scholars Program
- Caltech
- CZI Ben Barres Early Career Acceleration Award
Research shows that RNA and DNA play crucial roles in the spatial organization within the nucleus. Through the use of RD-SPRITE technology, the connections between RNA and chromatin structures have been revealed, providing a comprehensive analysis of nuclear functions.
RNA, DNA, and protein molecules are highly organized within three-dimensional (3D) structures in the nucleus. Although RNA has been proposed to play a role in nuclear organization, exploring this has been challenging because existing methods cannot measure higher-order RNA and DNA contacts within 3D structures. To address this, we developed RNA & DNA SPRITE (RD-SPRITE) to comprehensively map the spatial organization of RNA and DNA. These maps reveal higher-order RNA-chromatin structures associated with three major classes of nuclear function: RNA processing, heterochromatin assembly, and gene regulation. These data demonstrate that hundreds of ncRNAs form high-concentration territories throughout the nucleus, that specific RNAs are required to recruit various regulators into these territories, and that these RNAs can shape long-range DNA contacts, heterochromatin assembly, and gene expression. These results demonstrate a mechanism where RNAs form high-concentration territories, bind to diffusible regulators, and guide them into compartments to regulate essential nuclear functions.
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