4.6 Article

EZH2 knockout in oral cavity basal epithelia causes more invasive squamous cell carcinomas

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CARCINOGENESIS
卷 42, 期 12, 页码 1485-1495

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OXFORD UNIV PRESS
DOI: 10.1093/carcin/bgab091

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  1. National Institutes of Health [T32 CA062948, RO1 CA205258-S1, RO1 CA205258]
  2. Weill Cornell Medical College Funds

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Oral squamous cell carcinoma is an aggressive disease with low survival rates. Knockout of EZH2 in basal stem cells of tongue epithelia results in more aggressive carcinomas.
Oral squamous cell carcinoma (oral SCC) is an aggressive disease and despite intensive treatments, 5-year survival rates for patients have remained low in the last 20 years. Enhancer of zeste homolog 2 (EZH2), part of polycomb repressive complex 2 (PRC2), is highly expressed in human oral SCC samples and cell lines and has been associated with greater epithelia-to-mesenchymal transition (EMT), invasion and metastasis. Here, we developed a tamoxifen-regulated, transgenic mouse line (KcEZH2) in which EZH2 is selectively knocked out (KO) in some tongue epithelial basal stem cells (SCs) in adult mice. EZH2 KO SCs do not show the H3K27me3 mark, as assessed by double-label immunofluorescence. We used this mouse line to assess EZH2 actions during oral tumorigenesis with our immunocompetent 4-nitroquinoline 1-oxide model of oral SCC. We report that higher percentages of mice with invasive SCCs and high-grade neoplastic lesions are observed in mice containing EZH2 KO SCs (KcEZH2-2T. and KcEZH2-5T. mice). Moreover, EZH2 expression does not correlate with the expression of markers of invasive SCCs. Finally, EZH2 KO cells that are E-cadherin+ are present at invasion fronts infiltrating underlying muscle tissue. Our findings indicate that the knockout of EZH2 in basal SCs of tongue epithelia results in more aggressive carcinomas, and this should be considered when targeting EZH2 as a therapeutic strategy.

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