Expedited synthesis of mannose-6-phosphate containing oligosaccharides

Currently, the reaction toolbox for the functionalization of glycans assembled on solid-phase is quite limited. Automated (1 h) and manual (overnight) phosphorylation protocols that enable the solid-phase synthesis of oligosaccharides containing up to two mannose-6-phosphates are presented. Automated glycan assembly expedited access to substrates and facilitated the screening of experimental conditions.

Automated summary

The toolbox for the functionalization of glycans assembled on solid-phase is currently limited. This study presents automated and manual phosphorylation protocols for the solid-phase synthesis of oligosaccharides containing up to two mannose-6-phosphates. Automated glycan assembly allows fast access to substrates and facilitates the screening of experimental conditions.