Discrete genetic loci in human gutBacteroides thetaiotaomicron confer pectin metabolism

Although the polysaccharide utilization loci (PULs) activated by pectin have been defined, due to the complex of side-chain structure, the degradative mechanisms still remain vague. Thus, we hypothesize that there may have other specific PULs to target pectin. Here, we characterize loci-encoded proteins expressed by Bacteroides thetaiotaomicron (BT) that are involved in the pectin capturing, importation, de-branching and degradation into monosaccharides. Totally, four PULs contain ten enzymes and four glycan binding proteins which including a novel surface enzyme and a surface glycan binding protein are identified. Notably, PUL2 and PUL3 have not been reported so far. Further, we show that the degradation products support the growth of other Bacteroides spp. and probiotics. In addition, genes involved in this process are conservative in other Bacteroides spp. Our results further highlight the contribution of Bacteroides spp. to metabolism the pectic network.

Automated summary

Our study characterized proteins involved in pectin degradation by Bacteroides thetaiotaomicron, identifying specific PULs and enzymes responsible for capturing, importing, de-branching, and degrading pectin into monosaccharides. This contributes to understanding the role of Bacteroides spp. in metabolizing the pectic network and supporting the growth of other Bacteroides spp. and probiotics.