期刊
CARBOHYDRATE POLYMERS
卷 277, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.118894
关键词
Shrimp chaff; Co-fermentation; Chitosanase; Cell surface display; Chitosan; COS production
资金
- National Natural Science Foundation of China [31870082, 32070117, 31600079]
- Lanzhou Science and Technology Plan Project [2019-4-40]
- Natural Science Foundation of Gansu Province [20JR5RA234]
- Fundamental Research Funds for the Central Universities [lzujbky-2020-kb05]
A novel high-yield method utilizing cell surface-display CHI-1 was developed to produce chitooligosaccharides from shrimp chaff through co-fermentation with Bacillus subtilis and Acetobacter sp. The engineered E. coli BL21-pET23b(+)-NICHI exhibited excellent stability and maintained high hydrolysis efficiency over time. This proposed method showed promising potential for producing COS using whole engineered cells.
Chitin refers to a natural biopolymer, which is economically significant to next-generation biorefineries. In this study, a novel high-yield method with cell surface-display chitosanase (CHI-1) was built to produce chitooligosaccharides (COS) from shrimp chaff through the co-fermentation in the presence of Bacillus subtilis and Acetobacter sp. Under the optimized co-fermentation conditions (5 g/L yeast extracts, 10 g/L KH2PO4, 6% ethanol, 50 g/L glucose), the final deproteinization (DP) and demineralization (DM) efficiency and the chitin yield were achieved as 94, 92 and 18%, respectively. The engineered E. coli BL21-pET23b(+)-NICHI maintained 81% of the initial enzyme activity after 40 days at room temperature. The crude CHI-1 was inactivated after one day interacting with prepared chitosan. Moreover, E. coli BL21-pET23b(+)-NICHI still maintained excellent hydrolysis ability in 7 days, and the COS yield reached 41%. Accordingly, the proposed method exhibited excellent stability and a high hydrolysis efficiency to produce COS with whole engineered cells.
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