期刊
CARBOHYDRATE POLYMERS
卷 277, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.118791
关键词
Chitosan nanoparticles; Silk fibroin; microRNA-222; Nanofibrous scaffolds; Neuronal differentiation
资金
- National Natural Science Foundation of China [52073220, 51803160]
- National Key Research and Development Program of China [2018YFB1105500]
- Major Special Projects of Technological Innovation of Hubei Province [2017ACA168]
- Young TopNotch Talents Fund of Wuhan University of Tech-nology [471-40120093]
The miR-222/CS NPs effectively enhance neuronal differentiation of NSCs, suggesting a promising approach for neural tissue regeneration.
Neural stem cells (NSCs) transplantation therapy is a promising method for neural tissue regeneration. How to enhance the neuronal differentiation of NSCs has been the most challenging aspect of NSCs application. Herein, the microRNA-222 loaded chitosan nanoparticles (miR-222/CS NPs) were incorporated with silk fibroin (SF) nanofibrous scaffolds to enhance neuronal differentiation of NSCs. The encapsulation efficiency of miR-222 in the miR-222/CS NPs was (96.4 +/- 0.3) %. The results of the electrophoretic assay and cellular uptake assay confirmed that miR-222 was stable in the miR-222/CS NPs and can be effectively delivered into NSCs. The water contact angle decreased from (89 +/- 3.05)degrees for the SF scaffolds to (14 +/- 1.00)degrees for the composite scaffolds. The Western blot and RT-PCR results confirmed that the composite scaffolds could enhance neuronal differentiation of NSCs. In conclusion, the SF nanofibrous scaffolds in combination with miR-222/CS NPs are a promising approach for neural tissue regeneration.
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