期刊
CARBOHYDRATE POLYMERS
卷 277, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.118771
关键词
Endoglucanase; Blue mussel; Cel45A; GH45; Beta-glucan; Glucomannan
资金
- Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Formas) [2017-01130]
- Swedish Natural Science Research Council (NFR)
- Royal Swedish Academy of Sciences (KVA)
- Swedish Structural Biology Network (SBNet)
- Japanese Ministry of Education, Culture, Sports and Technol-ogy (MEXT) [18H05494]
- Grants-in-Aid for Scientific Research [18H05494] Funding Source: KAKEN
- Formas [2017-01130] Funding Source: Formas
In this study, the enzymatic hydrolysis of barley beta-glucan, konjac glucomannan, and carboxymethyl cellulose by MeCel45A from blue mussel was compared with GH45 members from different subfamilies, showing that subfamily B and C enzymes exhibited mannanase activity while the subfamily A member was uniquely able to produce monomeric glucose. MeCel45A appears to be cold adapted and maintained higher activity at 4 degrees Celsius compared to other enzymes.
The enzymatic hydrolysis of barley beta-glucan, konjac glucomannan and carboxymethyl cellulose by a beta-1,4-Dendoglucanase MeCel45A from blue mussel, Mytilus edulis, which belongs to subfamily B of glycoside hydrolase family 45 (GH45), was compared with GH45 members of subfamilies A (Humicola insolens HiCel45A), B (Trichoderma reesei TrCel45A) and C (Phanerochaete chrysosporium PcCel45A). Furthermore, the crystal structure of MeCel45A is reported. Initial rates and hydrolysis yields were determined by reducing sugar assays and product formation was characterized using NMR spectroscopy. The subfamily B and C enzymes exhibited mannanase activity, whereas the subfamily A member was uniquely able to produce monomeric glucose. All enzymes were confirmed to be inverting glycoside hydrolases. MeCel45A appears to be cold adapted by evolution, as it maintained 70% activity on cellohexaose at 4 degrees C relative to 30 degrees C, compared to 35% for TrCel45A. Both enzymes produced cellobiose and cellotetraose from cellohexaose, but TrCel45A additionally produced cellotriose.
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