期刊
CARBOHYDRATE POLYMERS
卷 276, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.118790
关键词
Bacterial cellulose; Decellularized extracellular matrix; Cartilage regeneration; Cartilage repair
资金
- National Natural Science Foundation of China [31870963]
- Ningbo Natural Science Foundation [2019A610240]
The study successfully developed 3D hierarchical porous BC/DCECM scaffolds with biomimetic cartilage regeneration microenvironment, which showed enhanced cell adhesion and proliferation. The scaffolds achieved satisfactory neocartilage tissue regeneration in both in vitro and in vivo experiments, demonstrating their potential for further application in cartilage tissue engineering.
The limited three-dimensional (3D) nano-scale pore structure and lack of biological function hamper the application of bacterial cellulose (BC) in cartilage tissue engineering. To address this challenge, 3D hierarchical porous BC/decellularized cartilage extracellular matrix (DCECM) scaffolds with structurally and biochemically biomimetic cartilage regeneration microenvironment were fabricated by freeze-drying technique after EDC/NHS chemical crosslinking. The BC/DCECM scaffolds exhibited excellent mechanical properties, water superabsorbency and shape-memory properties. Compared with the BC control, the BC/DCECM scaffolds exhibited enhanced cell adhesion and proliferation. Cartilage regeneration in vitro and in vivo indicated that the BC/DCECM scaffolds achieved satisfactory neocartilage tissue regeneration with superior original shape fidelity, exterior natural cartilage-like appearance and histologically cartilage-specific lacuna formation and ECM deposition. Furthermore, the BC/DCECM scaffolds achieved superior repair outcomes, as hyaline cartilage-like tissue formed within the defect sites. The present study constitutes a strong step toward the further application of BC in cartilage tissue engineering.
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