Monooxygenase System and NO Metabolism in Liver Microsomes of Rats with Toxic Hepatitis and the Effect of Sesquiterpene Lactones

We analyzed changes in activities of enzymes of phases I and II of xenobiotic biotransformation and parameters of NO metabolism in liver microsomes of rats with toxic CCl4-induced hepatitis after a 14-day course of sesquiterpene lactones from Artemisia leucodes (10 mg/kg). It was found that toxic hepatitis was associated with significant inhibition of NADPH-cytochrome c-reductase, benzo(a)pyrene hydroxylase, and NADPH-diaphorase, reduced cytochrome P-450 content, and enhanced induction of nitrate/nitrite reductase with accumulation of NO metabolites in the liver. Administration of sesquiterpene lactones stimulated activity of the studied components of the cytochrome P-450 system and promoted recovery of the NOergic system components; the effects were most pronounced in 7 and 14 days after treatment.

Automated summary

After a 14-day course of sesquiterpene lactones from Artemisia leucodes (10 mg/kg) in rats with toxic CCl4-induced hepatitis, significant changes in enzyme activities of phases I and II of xenobiotic biotransformation and parameters of NO metabolism were observed in liver microsomes, suggesting that sesquiterpene lactones can stimulate the recovery of the cytochrome P-450 system and NOergic system.