期刊
BRITISH JOURNAL OF CANCER
卷 126, 期 6, 页码 927-936出版社
SPRINGERNATURE
DOI: 10.1038/s41416-021-01652-y
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资金
- OU Center for Biomedical Research grant
- OU Provost grant
- NSF-MRI [NSF-1919572]
The study revealed that CSF-1 signaling induced differentiation of intratumoural HSPCs into M2 polarized TAMs, promoting post-RT tumour survival and regrowth. Conversely, hyperfractionated/pulsed radiation therapy (PRT) and GW2580 disrupted this process, resulting in improved tumour killing and mouse survival.
Background Bone-marrow-derived haematopoietic stem and progenitor cells (HSPCs) are a prominent part of the highly complex tumour microenvironment (TME) where they localise within tumours and maintain haematopoietic potency. Understanding the role HSPCs play in tumour growth and response to radiation therapy (RT) may lead to improved patient treatments and outcomes. Methods We used a mouse model of non-small cell lung carcinoma where tumours were exposed to RT regimens alone or in combination with GW2580, a pharmacological inhibitor of colony stimulating factor (CSF)-1 receptor. RT-PCR, western blotting and immunohistochemistry were used to quantify expression levels of factors that affect HSPC differentiation. DsRed(+) HSPC intratumoural activity was tracked using flow cytometry and confocal microscopy. Results We demonstrated that CSF-1 is enhanced in the TME following exposure to RT. CSF-1 signaling induced intratumoural HSPC differentiation into M2 polarised tumour-associated macrophages (TAMs), aiding in post-RT tumour survival and regrowth. In contrast, hyperfractionated/pulsed radiation therapy (PRT) and GW2580 ablated this process resulting in improved tumour killing and mouse survival. Conclusions Tumours coopt intratumoural HSPC fate determination via CSF-1 signaling to overcome the effects of RT. Thus, limiting intratumoural HSPC activity represents an attractive strategy for improving the clinical treatment of solid tumours.
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