期刊
BRITISH JOURNAL OF ANAESTHESIA
卷 127, 期 6, 页码 929-941出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.bja.2021.08.028
关键词
cognitive impairment; neuroprotection; sevoflurane; sex differences; tau phosphorylation; testosterone
资金
- National Institutes of Health [HD086977, AG070761]
The study showed that lower brain testosterone concentrations in neonatal male mice compared to adult male mice contributed to age-dependent tau phosphorylation and cognitive impairment after sevoflurane anesthesia. Testosterone treatment increased brain testosterone concentrations and alleviated anesthesia-induced tau phosphorylation and cognitive impairment.
Background: Sevoflurane anaesthesia induces phosphorylation of the microtubule-associated protein tau and cognitive impairment in neonatal, but not adult, mice. The underlying mechanisms remain largely to be determined. Sex hormones can be neuroprotective, but little is known about the influence of testosterone on age-dependent anaesthesia effects. Methods: Six-and 60-day-old male mice received anaesthesia with sevoflurane 3% for 2 h daily for 3 days. Morris water maze, immunoassay, immunoblotting, co-immunoprecipitation, nanobeam technology, and electrophysiology were used to assess cognition; testosterone concentrations; tau phosphorylation; glycogen synthase kinase-3I3 (GSK3I3) activation; binding or interaction between tau and GSK3 beta; and neuronal activation in mice, cells, and neurones. Results: Compared with 60-day-old male mice, 6-day-old male mice had lower testosterone concentrations (3.03 [0.29] vs 0.44 [0.12] ng ml(-1); P<0.01), higher sevoflurane-induced tau phosphorylation in brain (133 [20]% vs 100 [6]% in 6-day-old mice, P<0.01; 103 [8]% vs 100 [13]% in 60-day-old mice, P=0.77), and sevoflurane-induced cognitive impairment. Testosterone treatment increased brain testosterone concentrations (1.76 [0.10] vs 0.39 [0.05] ng ml(-1); P<0.01) and attenuated the sevoflurane-induced tau phosphorylation and cognitive impairment in neonatal male mice. Testosterone inhibited the interaction between tau and GSK3 beta, and attenuated sevoflurane-induced inhibition of excitatory postsynaptic currents in hippocampal neurones. Conclusions: Lower brain testosterone concentrations in neonatal compared with adult male mice contributed to age dependent tau phosphorylation and cognitive impairment after sevoflurane anaesthesia. Testosterone might attenuate the sevoflurane-induced tau phosphorylation and cognitive impairment by inhibiting the interaction between tau and GSK3 beta.
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