期刊
BRAZILIAN JOURNAL OF MICROBIOLOGY
卷 53, 期 1, 页码 317-326出版社
SPRINGER
DOI: 10.1007/s42770-021-00631-y
关键词
Enteric virus; Estuarine; Food safety; Mussels; NoV; Oysters
类别
资金
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
The study found that AstV virus was not detected in any shellfish samples, while NoV GII virus was found in 21 out of 150 samples, with 8 mussel samples and 13 oyster samples testing positive. Brazilian samples were grouped with sequences from Brazil, Japan, and Mexico in molecular characterization.
In recent years, annual cases of gastroenteritis have been reported in the world at high rates, suggesting an association with the consumption of shellfish with enteric viruses in their tissues. Anthropic activities are considered a source of environmental pollution and the main responsible for contamination by pathogenic microorganisms in aquatic environments. The objective of this study was to evaluate, by RT-semi-nested PCR, the presence of astrovirus (AstV) and norovirus genogroup II (NoV GII) in mussels (Mytella falcata) and oysters (Crassostrea brasiliana) collected in two sites of the Lagunar Complex of Cananeia, State of Sao Paulo, Brazil. A total of 150 samples of mussels and oysters (75 samples each) were analyzed. AstV was not identified in any shellfish sample. NoV GII was detected in 21 samples (14%), 8 mussel samples (38%), and 13 oyster samples (62%). From the 21 positive samples, 16 were analyzed by nucleotide sequencing. The molecular characterization revealed that Brazilian samples were grouped into clades along with other sequences from Brazil, Japan, and Mexico. There was 93.8-100% amino acid sequence similarity among the samples in this study and > 94.9% when compared with the strains isolated from clinical cases in Brazil. The screening of shellfish for the presence of health-significant enteric viruses can help prevent outbreaks among consumers and contribute to the improvement of the estuarine environment.
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