Antimicrobial effect of Casiopeinas® copper- and ruthenium-based compounds on Aggregatibacter actinomycetemcomitans and in vitro cell viability onto osteoblasts cells

Objectives The present study aims to evaluate the antimicrobial property of Casiopeinas (R) copper- and ruthenium-based compounds against Aggregatibacter actinomycetemcomitans serotype b (ATCC (R) 43,718 (TM)), as well as the cytotoxicity on an osteoblasts cell line of both compounds. Material and methods The antibacterial effect of the copper-based compounds (CasII-gly, CasIII-ia) and the ruthenium-based compound (RuN-6) at four different concentrations was evaluated as the inhibition ratio of the bacterial growth after 48 h under anaerobic conditions, and the cell viability was measured through resazurin assay. Results The copper- and ruthenium-based compounds used for this assay were (CasII-gly, CasIII-ia, and RuN-6), showing inhibitory activity between 39 and 62% compared to the antibiotic employed as control 66%. Cell viability was established between 61 and 96%. Conclusions Casiopeinas (R) and ruthenium showed dose and time dependent, inhibitory activity on A. actinomycetemcomitans, and low toxicity on cells (osteoblast) underexposure. The compound CasII-gly showed the best antimicrobial effect, and it could be considered a possible antimicrobial agent in periodontal therapy.

Automated summary

The study evaluated the antimicrobial properties of Casiopeinas (R) and ruthenium-based compounds against Aggregatibacter actinomycetemcomitans, showing inhibitory activity and low toxicity on osteoblast cells. Among the compounds tested, CasII-gly exhibited the best antimicrobial effect and could be considered as a potential antimicrobial agent in periodontal therapy.