4.7 Article

The endogenous inflammatory reflex inhibits the inflammatory response to different immune challenges in mice

期刊

BRAIN BEHAVIOR AND IMMUNITY
卷 97, 期 -, 页码 371-375

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbi.2021.07.019

关键词

Inflammatory reflex; Splanchnic anti-inflammatory pathway; Sympathetic nervous system; Inflammation; Lipopolysaccharide (LPS); Dipalmitoyl-S-glyceryl cysteine (Pam2cys); Toll-like receptor (TLR); Tumor necrosis factor alpha (TNF); Interleukin 10 (IL-10)

资金

  1. National Health and Medical Research Council (NHMRC) of Australia [1098887]
  2. Victorian Government Operational Infrastructure Support Program
  3. National Health and Medical Research Council of Australia [1098887] Funding Source: NHMRC

向作者/读者索取更多资源

The splanchnic anti-inflammatory pathway suppresses inflammatory response in mice and can be activated by various inflammatory stimuli, indicating its potential therapeutic benefits in managing inflammatory conditions.
The splanchnic anti-inflammatory pathway, the efferent arm of the endogenous inflammatory reflex, has been shown to suppress the acute inflammatory response of rats to systemic lipopolysaccharide (LPS). Here we show for the first time that this applies also to mice, and that the reflex may be engaged by a range of inflammatory stimuli. Experiments were performed on mice under deep anaesthesia. Half the animals were subjected to bilateral section of the splanchnic sympathetic nerves, to disconnect the splanchnic anti-inflammatory pathway, while the remainder underwent a sham operation. Mice were then challenged intravenously with one of three inflammatory stimuli: the toll-like receptor (TLR)-4 agonist, LPS (60 mu g/kg), the TLR-3 agonist Polyinosinic: polycytidylic acid (Poly I:C, 1 mg/kg) or the TLR-2 and -6 agonist dipalmitoyl-S-glyceryl cysteine (Pam2cys, 34 mu g/kg). Ninety minutes later, blood was sampled by cardiac puncture for serum cytokine analysis. The splanchnic anti-inflammatory reflex action was assessed by comparing cytokine levels between animals with cut versus those with intact splanchnic nerves. A consistent pattern emerged: Tumor necrosis factor (TNF) levels in response to all three challenges were raised by prior splanchnic nerve section, while levels of the anti-inflammatory cytokine interleukin 10 (IL-10) were reduced. The raised TNF:IL-10 ratio after splanchnic nerve section indicates an enhanced inflammatory state when the reflex is disabled. These findings show for the first time that the inflammatory reflex drives a coordinated anti-inflammatory action also in mice, and demonstrate that its antiinflammatory action is engaged, in similar fashion, by inflammatory stimuli mimicking a range of bacterial and viral infections.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据