4.6 Article

Molecular characterization of African swine fever viruses from Burkina Faso, 2018

期刊

BMC VETERINARY RESEARCH
卷 18, 期 1, 页码 -

出版社

BMC
DOI: 10.1186/s12917-022-03166-y

关键词

African swine fever; Central variable region; B646L; B602L; E183L; Burkina Faso

资金

  1. VETLAB network initiative of the Joint FAO/IAEA Division

向作者/读者索取更多资源

The outbreak of African swine fever (ASF) in central Burkina Faso was caused by African swine fever virus (ASFV) isolates belonging to genotype I and serogroup 4. The study also revealed the existence of multiple variants, emphasizing the importance of molecular characterization in understanding the evolution of ASFV isolates and the link between epidemics.
Background African swine fever (ASF) is a viral hemorrhagic disease of domestic and wild swine. ASF has been endemic in Burkina Faso since 2003. In October 2018, substantial pig deaths occurred in Ouagadougou and two neighboring municipalities in central Burkina Faso. Following these mortalities, the veterinary extension services carried out investigations to begin control measures and collect samples. Methods We performed real-time PCR for diagnostic confirmation and molecular characterization of the virus based on the partial P72, the complete p54, the partial CD2v, and partial B602L genes. Results The field study revealed that mortalities started two weeks before our investigations. The real-time PCR results confirmed ASFV DNA in twenty samples out of sixty-two blood samples collected in four different locations. The sequencing and phylogenetic analysis showed that ASFVs causing these outbreaks belong to genotype I and serogroup 4. The study of the CVR showed 4 TRS variants, and that of the CD2v amino acid sequence revealed five variants based on the number of deleted KCPPPK motifs in the C-terminal proline-reach region of the protein. Conclusions The existence of multiple variants in these outbreaks shows the importance of molecular characterization to understand the evolution of ASFV isolates and the link between epidemics.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据