期刊
BMC VETERINARY RESEARCH
卷 18, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12917-022-03137-3
关键词
Extracellular vesicles; Mares; Endometritis; Uterine lavage; Serum; Interleukin 6; Prostaglandins
资金
- Science and Technology Development Fund (STDF) [33342]
This study investigated the role of EVs in mares with endometritis. The results showed that mares with endometritis released higher amounts of larger size EVs, which differentially expressed CD9 and CD63. In addition, mares suffering from endometritis evoked higher levels of inflammatory mediators.
Background Extracellular vesicles (EVs) are a promising biomarker and play a vital role in cell-cell communication. This study aimed (I) to identify and characterize EVs from low volume uterine lavage (LVL) and serum in mares with endometritis, compared to healthy controls and (II) to measure serum levels of interleukin 6 (IL-6), and prostaglandins (PGF(2 alpha) and PGE(2)). Mares were divided into 30 sub-fertile (endometritis) and 20 fertile (controls). Serum and LVL was collected for EV isolation, and determination of serum levels of inflammatory mediators. Characterization and visualization of EVs were done by electron microscopy, dynamic light scattering and flow cytometry. Results Serial ultracentrifugation of LVL and use of a commercial kit for serum were strategies for EVs isolation. Mares with endometritis released higher amounts of larger size EVs. The EVs from mares with endometritis differentially expressed CD9 and CD63, compared to controls. Mares suffering from endometritis evoked higher levels of inflammatory mediators. Conclusions Thus, EVs could be used for a better understanding the regulatory mechanisms associated with developing endometritis in mares.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据