期刊
BMC MICROBIOLOGY
卷 22, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12866-022-02438-9
关键词
Antimicrobial photodynamic therapy; Antimicrobial sonodynamic therapy; Biofilms; Burn wound infection; Curcumin; Nisin; Silver sulfadiazine
类别
资金
- Tehran University of Medical Science and Health Services [1399-4-45939]
The study demonstrated that CurNisNp-mediated aPSDT may be a promising complementary approach to treat burn wound infections by reducing biofilm growth and promoting wound healing.
Background The biofilm-forming ability of Acinetobacter baumannii in the burn wound is clinically problematic due to the development of antibiotic-resistant characteristics, leading to new approaches for treatment being needed. In this study, antimicrobial photo-sonodynamic therapy (aPSDT) was used to assess the anti-biofilm efficacy and wound healing activity in mice with established A. baumannii infections. Methods Following synthesis and confirmation of Curcumin-Nisin-based poly (L-lactic acid) nanoparticle (CurNisNp), its cytotoxic and release times were evaluated. After determination of the sub-significant reduction (SSR) doses of CurNisNp, irradiation time of light, and ultrasound intensity against A. baumannii, anti-biofilm activity and the intracellular reactive oxygen species (ROS) generation were evaluated. The antibacterial and anti-virulence effects, as well as, histopathological examination of the burn wound sites of treated mice by CurNisNp-mediated aPSDT(SSR) were assessed and compared with silver sulfadiazine (SSD) as the standard treatment group. Results The results showed that non-cytotoxic CurNisNp has a homogeneous surface and a sphere-shaped vesicle with continuous release until the 14th day. The dose-dependent reduction in cell viability of A. baumannii was achieved by increasing the concentrations of CurNisNp, irradiation time of light, and ultrasound intensity. There was a time-dependent reduction in biofilm growth, changes in gene expression, and promotion in wound healing by the acceleration of skin re-epithelialization in mice. Not only there was no significant difference between aPSDT(SSR) and SSD groups in antibacterial and anti-virulence activities, but also wound healing and re-epithelialization occurred more efficiently in aPSDT(SSR) than in the SSD group. Conclusions In conclusion, CurNisNp-mediated aPSDT might be a promising complementary approach to treat burn wound infections.
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