期刊
BMC INFECTIOUS DISEASES
卷 22, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12879-022-07074-2
关键词
HCV; Genotype; Core antigen; Transmission; Hemodialysis; Dialyzer reuse
资金
- Thailand Research Fund (TRF) [RTA6280004]
- Center of Excellence in Hepatitis and Liver Cancer, Faculty of Medicine, Chulalongkorn University
This study investigated the prevalence and genotypes of HCV in multiple hemodialysis facilities in Thailand and evaluated the accuracy of HCVcAg testing for viremia. The results showed that the HCV infection rate and subtypes in hemodialysis centers were different from the general population, and possible HCV transmission within the same centers was identified. The findings emphasize the importance of enhancing preventive measures, dialyzer reuse policy, and better access to antiviral therapy for achieving HCV micro-elimination within hemodialysis facilities.
Background Individuals with end-stage renal disease have a higher risk of hepatitis C virus (HCV) acquisition during long-term hemodialysis (HD). Our report was designed to investigate HCV prevalence and genotype, in addition to the clinical use of HCV core antigen (HCVcAg), within multiple HD facilities in Thailand. Methods This cross-sectional report was investigated between January and June 2019. HCV infection was assessed by anti-HCV and confirmed active infection by measuring HCV RNA and HCVcAg. HCV genotype was determined by phylogenetic analysis using nucleotide sequences of NS5B region. Results Overall, 140 of 3,305 (4.2%) patients in 15 dialysis centers had anti-HCV positive. Among them, HCV RNA was further assessed in 93 patients and was detectable in 59 (63.4%) persons. Considering HCV viremia, HCVcAg measurement exhibited high accuracy (96.8%), sensitivity (94.9%) and specificity (100%) in comparison with HCV RNA testing. Moreover, individuals infected with HCV received a longer duration of dialysis vintage when compared to anti-HCV negative controls. The major sub-genotypes were 1a, 1b, 3a, 3b, 6f and 6n. Regarding phylogenetic analysis, there were 7 clusters of isolates with high sequence homology affecting 17 individuals, indicating possible HCV transmission within the same HD centers. Conclusions HCV frequency and common sub-genotypes in HD centers were different from those found in the Thai general population. HCVcAg might be an alternate testing for viremia within resource-limited countries. Enhanced preventive practices, dialyzer reuse policy and better access to antiviral therapy are crucial for HCV micro-elimination within HD facilities.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据