期刊
BMC BIOLOGY
卷 19, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12915-021-01161-7
关键词
MIEF1; 2; Mfn1; 2; hFis1; Drp1; Mitochondrial dynamics; Mitochondrial fusion; Mitochondrial fission
类别
资金
- Swedish Research Council
- Swedish Cancer Society
- Cancer Society in Stockholm
- Karolinska Institutet
- Stockholm County Council
- Merck AG
- Swedish Research Council [2018-03102]
- Swedish Research Council [2018-03102] Funding Source: Swedish Research Council
Our study reveals that MIEFs play a key role in regulating both fission and fusion machineries by interacting with fusion proteins Mfn1 and Mfn2, providing a novel mechanism for balancing mitochondrial dynamics in mammals.
Background Mitochondrial dynamics is the result of a dynamic balance between fusion and fission events, which are driven via a set of mitochondria-shaping proteins. These proteins are generally considered to be binary components of either the fission or fusion machinery, but potential crosstalk between the fission and fusion machineries remains less explored. In the present work, we analyzed the roles of mitochondrial elongation factors 1 and 2 (MIEF1/2), core components of the fission machinery in mammals. Results We show that MIEFs (MIEF1/2), besides their action in the fission machinery, regulate mitochondrial fusion through direct interaction with the fusion proteins Mfn1 and Mfn2, suggesting that MIEFs participate in not only fission but also fusion. Elevated levels of MIEFs enhance mitochondrial fusion in an Mfn1/2- and OPA1-dependent but Drp1-independent manner. Moreover, mitochondrial localization and self-association of MIEFs are crucial for their fusion-promoting ability. In addition, we show that MIEF1/2 can competitively decrease the interaction of hFis1 with Mfn1 and Mfn2, alleviating hFis1-induced mitochondrial fragmentation and contributing to mitochondrial fusion. Conclusions Our study suggests that MIEFs serve as a central hub that interacts with and regulates both the fission and fusion machineries, which uncovers a novel mechanism for balancing these opposing forces of mitochondrial dynamics in mammals.
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