期刊
BIOTECHNOLOGY JOURNAL
卷 17, 期 2, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.202100478
关键词
in situ separation; multiphase system; real-time; sophorolipids; ultrasonic enhanced sedimentation
资金
- National Key Research Development Program of China [2019YFA0904803]
This study analyzed the sedimentation mechanism of Sophorolipids (SLs) and introduced ultrasonic enhanced sedimentation technology (UEST) to increase SLs productivity and yield, while reducing loss ratio. This provides a new horizon for optimizing the SLs fermentation process.
Background Sophorolipids (SLs) are regarded as one of the most promising biosurfactants. However, high production costs are the main obstacle to extended SLs application. Semi-continuous fermentation, which is based on in situ separation, is a promising technology for achieving high SLs productivity. Methods and Results In this study, the sedimentation mechanism of SLs was analyzed. The formation of a hydrophobic mixture of SLs and rapeseed oil was a key factor in sedimentation. And the hydrophobicity and density of the mixture determined SLs sedimentation rate. On this basis, ultrasonic enhanced sedimentation technology (UEST) was introduced, by which the sedimentation rates were increased by 46.9%-485.4% with different ratio of rapeseed oil to SLs. UEST-assisted real-time in situ separation and semi-continuous fermentation were performed. SLs productivity and yield were 2.15 g L-1 h(-1) and 0.58 g g(-1), respectively, simultaneously the loss ratio of cells, glucose, and rapeseed oil were significantly reduced. Conclusions This study provides a new horizon for optimization of the SLs fermentation process.
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